美國戴安Dionex AminoPac PA1 Amino Acid Column 037022 生物色譜柱
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產(chǎn)品名稱: 美國戴安Dionex AminoPac PA1 Amino Acid Column 037022 生物色譜柱
產(chǎn)品型號(hào): 037022
產(chǎn)品展商: 戴安Dionex
產(chǎn)品文檔: 無相關(guān)文檔
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美國戴安Dionex AminoPac PA1 Amino Acid Column 037022 生物色譜柱
的詳細(xì)介紹
AminoPac PA1 Amino Acid Column
For Specialized Amino Acid Applications with Postcolumn Derivatization
The AminoPac? PA1 column is a high-speed, pellicular, strong, anion-exchange column.
The PA1 delivers high-efficiency separations as a result of its
nonporous-core particle. Its strong anion-exchange characteristics are
imparted by agglomerating surface MicroBead? particles that are
functionalized with the quaternary ammonium functional group, resulting
in a higher capacity than typically offered by nonporous resins.
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Resolves phosphorylated and other amino acids by anion-exchange chromatography
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Separates acid-labile amino acids under basic pH conditions
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Separates strongly acidic amino acids
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Requires OPA or ninhydrin postcolumn derivatization
The
anion-exchange selectivity of the AminoPac PA1 can be used to resolve
peaks that cannot be resolved using a cation-exchange column. For
example, O-phosphorylated amino acids are resolved and eluted near the
end of a hydrolysate chromatogram. Arogenic acid, a precursor to
tyrosine and phenylalanine, decomposes under the acidic conditions
required for cation-exchange separation or PITC derivatization, but it
is stable in alkaline eluents used with AminoPac PA1 column.
The
PA1 is particularly well suited for acidic and acid-labile amino acids
and for amino acid pairs not completely resolved by cation-exchange
chromatography.
AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization Specifications
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Dimensions
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AminoPac PA1 Analytical Column:
4 × 250 mm
AminoPac PA1 Guard Column:
4 × 50 mm
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Maximum operating pressure
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4000 psi (27.9 MPa)
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Recommended operating temperature
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Ambient
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Typical operating conditions
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1.0 mL/min gradient
Recommended flow rate: 1.0 mL/min
|
Mobile phase compatibility
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Compatible with up to 5% common organic solvents.
Recommended eluents: hydroxide, borate, and acetate.
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Resin composition
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10-μm-diameter, pellicular substrate (2% cross-linked)
agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked).
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Anion-exchange capacity
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N/A
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Column construction
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PEEK with 10-32 threaded ferrule-style end fittings.
All components are nonmetallic.
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Analytical Columns
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AminoPac PA1 Analytical Column (4 x 250 mm)
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037022
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Guard Columns
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AminoPac PA1 Guard Column (4 x 50 mm)
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037023
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